5XOR
Crystal structure of N-terminal replicase protein of porcine circovirus type 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.932, 71.213, 131.718 |
Unit cell angles | 90.00, 104.34, 90.00 |
Refinement procedure
Resolution | 42.340 - 2.699 |
R-factor | 0.2306 |
Rwork | 0.224 |
R-free | 0.28870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hw0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.154 |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 46536 |
<I/σ(I)> | 12.2 |
Completeness [%] | 97.9 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 0.1M HEPES sodium pH 8.0, 0.45 M sodium citrate tribasic dihydrate, 50% (+/-)-2-Methyl-2,4-pentanediol |