5XFN
Structure of the N-terminal domains of PHF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2014-10-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.025, 62.000, 135.368 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.483 - 1.900 |
| R-factor | 0.1848 |
| Rwork | 0.183 |
| R-free | 0.21790 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.998 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.135 | 0.849 |
| Number of reflections | 26861 | |
| <I/σ(I)> | 16.6 | 1.9 |
| Completeness [%] | 97.3 | 96.7 |
| Redundancy | 4.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Tris-pH 8.0, 10% PEG 3350, 22% ethylene glycerol |
