5XEC
Heterodimer constructed from PA cyt c551-HT cyt c552 and HT cyt c552-PA cyt c551 chimeric proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-12 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.350, 72.540, 35.010 |
Unit cell angles | 90.00, 106.07, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.160 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.078 | 0.389 |
Number of reflections | 60431 | 8875 |
<I/σ(I)> | 7.8 | 2.7 |
Completeness [%] | 99.3 | 100 |
Redundancy | 3.5 | 3.5 |
CC(1/2) | 0.997 | 0.843 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 100 mM Tris-HCl containing 200 mM sodium acetate, 30% w/v PEG 4000 |