5X9W
Mismatch Repair Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 89.510, 102.070, 235.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.930 - 3.300 |
R-factor | 0.2239 |
Rwork | 0.221 |
R-free | 0.27292 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e3m |
RMSD bond length | 0.011 |
RMSD bond angle | 1.330 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.480 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.127 | 0.575 |
Number of reflections | 32442 | |
<I/σ(I)> | 8.5 | 2.5 |
Completeness [%] | 98.9 | 98.9 |
Redundancy | 5.3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10-14% (w/v) PEG 4000, 0.1M MES buffer (pH 6.5), 10-14% (v/v) glycerol, 0.1-0.2 M MgCl2 |