5X3I
Kfla1895 D451A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-11 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 181.145, 181.145, 391.832 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.546 - 2.100 |
R-factor | 0.2046 |
Rwork | 0.204 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.106 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.550 |
High resolution limit [Å] | 2.100 |
Number of reflections | 143534 |
<I/σ(I)> | 22.2 |
Completeness [%] | 100.0 |
Redundancy | 20.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.8 M lithium sulfate, 0.1 M imidazole |