5WZE
The structure of Pseudomonas aeruginosa aminopeptidase PepP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97776 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.197, 123.432, 149.485 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.008 - 1.783 |
R-factor | 0.2106 |
Rwork | 0.210 |
R-free | 0.22050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1AZ9 |
RMSD bond length | 0.011 |
RMSD bond angle | 0.997 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.010 | 1.827 |
High resolution limit [Å] | 1.783 | 1.783 |
Rmerge | 0.709 | |
Number of reflections | 194517 | 18600 |
<I/σ(I)> | 19.714 | |
Completeness [%] | 99.0 | |
Redundancy | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 30% PEG400, 100 mM sodium cacodylate pH 6.5, 200 mM lithium sulfate |