5WUN
Crystal structure of mouse importin-alpha1 bound to non-phosphorylated NLS of EBNA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2016-11-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.510, 90.180, 98.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.800 - 2.200 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ukw |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.683 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.800 | 28.800 | 2.270 |
| High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
| Rmerge | 0.087 | 0.021 | 0.990 |
| Rmeas | 0.095 | ||
| Rpim | 0.038 | ||
| Total number of observations | 218370 | ||
| Number of reflections | 36317 | ||
| <I/σ(I)> | 14.1 | ||
| Completeness [%] | 99.9 | 97.1 | 99.4 |
| Redundancy | 6 | 5.2 | 5.2 |
| CC(1/2) | 0.998 | 0.995 | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | MES, sodium citrate, DTT |
