5WTQ
Crystal structure of human proteasome-assembling chaperone PAC4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2016-07-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.90000 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 72.548, 152.779, 212.911 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.207 |
Rwork | 0.204 |
R-free | 0.25400 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.868 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.087 | 0.800 |
Number of reflections | 46618 | |
<I/σ(I)> | 42 | 3.3 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.4 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.0 M lithium sulfate, 0.1 M Tris-HCl (pH 8.5), and 10 mM nickel chloride |