5WOU

Crystal Structure of drosophila melanogaster Scribble PDZ1 domain in complex with Guk-Holder

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	C 2 2 21
Unit cell lengths	36.429, 53.884, 94.219
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.179 - 1.550
R-factor	0.1981
Rwork	0.197
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vwc
RMSD bond length	0.003
RMSD bond angle	0.680
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.420	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.094	0.580
Rpim	0.031	0.230
Number of reflections	13578	617
<I/σ(I)>	18.4	4.3
Completeness [%]	98.2	92
Redundancy	13.8	13.4
CC(1/2)	0.990	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	30 % (v/v) PEG 4000, 0.2 M sodium acetate and 0.1M Tris chloride pH 8.5