5WO9

Crystal Structure of Transient Receptor Potential (TRP) channel TRPV6* in the presence of Calcium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.75
Spacegroup name	P 4 21 2
Unit cell lengths	145.480, 145.480, 110.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.664 - 3.700
R-factor	0.2737
Rwork	0.272
R-free	0.31580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iwp
RMSD bond length	0.003
RMSD bond angle	0.571
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.700
Number of reflections	24281
<I/σ(I)>	1.07
Completeness [%]	96.0
Redundancy	18.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	150 mM Nacl, 100 mM TRIS, 22% PEG 350