5WL7
Crystal structure of chalcone isomerase engineered from ancestral inference (ancCHI*)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.440, 65.440, 94.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.070 - 1.900 |
R-factor | 0.178 |
Rwork | 0.175 |
R-free | 0.20620 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.277 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.070 |
High resolution limit [Å] | 1.900 |
Number of reflections | 25236 |
<I/σ(I)> | 11.04 |
Completeness [%] | 100.0 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 4.5M NaCl, 50mM acetic acid |