5WIW
Crystal structure of murine 4-1BB N128A mutant from HEK293T cells in P43 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 43 |
| Unit cell lengths | 66.639, 66.639, 82.184 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.120 - 2.300 |
| R-factor | 0.1988 |
| Rwork | 0.197 |
| R-free | 0.22790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wjf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.220 |
| High resolution limit [Å] | 2.180 | 5.910 | 2.180 |
| Rmerge | 0.070 | 0.042 | 0.493 |
| Rmeas | 0.078 | 0.047 | 0.554 |
| Rpim | 0.033 | 0.020 | 0.247 |
| Total number of observations | 100598 | ||
| Number of reflections | 18619 | 964 | 920 |
| <I/σ(I)> | 6.1 | ||
| Completeness [%] | 99.4 | 99.3 | 97.9 |
| Redundancy | 5.4 | 5.3 | 4.7 |
| CC(1/2) | 0.999 | 0.821 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M sodium acetate pH 4.5, 30% w/V PEG 8000, 0.2M lithium sulfate |
