5WHU

Crystal structure of 3'SL bound ArtB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-18
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	137.915, 94.213, 123.964
Unit cell angles	90.00, 102.60, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.1767
Rwork	0.174
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rhr
RMSD bond length	0.007
RMSD bond angle	1.109
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	77088
<I/σ(I)>	8.5
Completeness [%]	98.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	297	PEG 200, calcium chloride