5WF1
Tepsin VHS/ENTHlike domain SeMet
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-10-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 65 |
Unit cell lengths | 58.775, 58.775, 69.522 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.387 - 1.954 |
R-factor | 0.1882 |
Rwork | 0.184 |
R-free | 0.22240 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.765 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rpim | 0.035 | 0.309 |
Number of reflections | 9904 | |
<I/σ(I)> | 28.14 | 2.25 |
Completeness [%] | 99.0 | 99.73 |
Redundancy | 6.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M Sodium tartrate dibasic dihydrate, 20% w/v Polyethylene glycol 3,350 |