5VZW

TRIM23 RING domain in complex with UbcH5-Ub

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-22
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	52.830, 109.355, 122.963
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.570 - 2.278
R-factor	0.2101
Rwork	0.208
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eya
RMSD bond length	0.003
RMSD bond angle	0.595
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.278	2.278
Rmerge	0.117	1.200
Rpim	0.033	0.440
Number of reflections	33080
<I/σ(I)>	20.1
Completeness [%]	99.9
Redundancy	13.7
CC(1/2)		0.645

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	0.1 M Bis-Tris, pH 5.5, 0.2 M calcium chloride, 17% w/v PEG3350