5VZG
Pre-catalytic ternary complex of human Polymerase Mu (W434H) mutant with incoming nonhydrolyzable UMPNPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-06 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.668, 68.555, 110.108 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.845 - 1.850 |
R-factor | 0.1767 |
Rwork | 0.175 |
R-free | 0.20150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.134 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rpim | 0.026 | 0.225 |
Number of reflections | 39094 | 1885 |
<I/σ(I)> | 20.85 | 2.54 |
Completeness [%] | 99.5 | 98.3 |
Redundancy | 5.1 | 2.9 |
CC(1/2) | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 90mM HEPES pH 7.5, 18% PEG4K |