5VZ9
Post-catalytic complex of human Polymerase Mu (G433A) mutant with incoming dTTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-04-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.190, 68.847, 111.355 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.145 - 1.650 |
R-factor | 0.1619 |
Rwork | 0.160 |
R-free | 0.19050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.001 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rpim | 0.030 | 0.369 |
Number of reflections | 56900 | 2827 |
<I/σ(I)> | 27.4 | 3.14 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.1 | 5.4 |
CC(1/2) | 0.806 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 50mM HEPES pH 7.5, 10% PEG4K |