5VZ1

Crystal structure of the Apo Antibody fragment (Fab) raised against C-terminal domain of Ebola nucleoprotein (EBOV, TAFV, BDBV strains)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-25
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.000
Spacegroup name	P 41 2 2
Unit cell lengths	74.621, 74.621, 323.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.310 - 2.112
R-factor	0.2203
Rwork	0.218
R-free	0.26750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vkd
RMSD bond length	0.002
RMSD bond angle	0.681
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.400
High resolution limit [Å]	2.110
Number of reflections	50593
<I/σ(I)>	4.23
Completeness [%]	93.7
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	90 mM imidazole, pH 8.0, 180 mM Zinc Acetate, 27% PEG 3000, 10 mM Chromium (III) chloride hexahydrate