5VNI
Crystal structure of Sec23a/Sec24a/Sec22 complexed with a C-terminal FA sorting motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2015-07-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.987 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 147.606, 96.065, 129.998 |
Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
Resolution | 48.803 - 2.788 |
R-factor | 0.2147 |
Rwork | 0.212 |
R-free | 0.26970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nut |
RMSD bond length | 0.002 |
RMSD bond angle | 0.481 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.803 | 2.900 |
High resolution limit [Å] | 2.788 | 2.788 |
Rmerge | 0.116 | 0.582 |
Rpim | 0.083 | 0.427 |
Number of reflections | 40031 | 3587 |
<I/σ(I)> | 12.6 | 1 |
Completeness [%] | 98.2 | 98.2 |
Redundancy | 2.5 | 2.5 |
CC(1/2) | 0.670 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50mM HEPES PH 7.8, 0.5M NaAc and 12% PEG4K |