5VN7
Structure of bacteriorhodopsin from crystals grown at 20 deg Celcius using GlyNCOC15+4 as an LCP host lipid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | H 3 |
| Unit cell lengths | 107.050, 107.050, 149.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.519 - 2.700 |
| R-factor | 0.1976 |
| Rwork | 0.196 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xji |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.646 |
| Data reduction software | XDS |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.138 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.151 | 0.645 |
| Number of reflections | 17181 | 1716 |
| <I/σ(I)> | 7.4 | 1.8 |
| Completeness [%] | 97.0 | 96 |
| Redundancy | 3 | 2.4 |
| CC(1/2) | 0.988 | 0.476 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.6 | 293 | 1.9-3.0 M sodium/potassium phosphate buffer (pH5.6), 3.5% v/v methylpentanediol and 0.5% w/v OG |
