5VK7
aspartate aminotransferase pH 4.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2017-01-27 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.732, 125.249, 131.116 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
Rwork | 0.185 |
R-free | 0.22500 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.052 | 0.376 |
Number of reflections | 69048 | 7027 |
<I/σ(I)> | 24.2 | 3.4 |
Completeness [%] | 94.4 | 97.9 |
Redundancy | 4.6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 5.4 | 277.15 | 40 mM sodium acetate 8% PEG 6,000 |