5VG3
Structure of Oxalate Decarboxylase from Bacillus subtilis at pH 4.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.484, 156.657, 79.574 |
Unit cell angles | 90.00, 119.28, 90.00 |
Refinement procedure
Resolution | 35.210 - 1.451 |
R-factor | 0.1551 |
Rwork | 0.154 |
R-free | 0.17670 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.862 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.210 | 1.503 |
High resolution limit [Å] | 1.451 | 1.451 |
Rmerge | 0.097 | 0.575 |
Rpim | 0.056 | 0.336 |
Number of reflections | 203372 | 20279 |
<I/σ(I)> | 8.78 | 2.11 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 4 | 3.9 |
CC(1/2) | 0.992 | 0.747 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1 M sodium acetate trihydrate, 0.2 M sodium chloride, 30% v/v MPD |