5VE8
Crystal structure of full-length Kluyveromyces lactis Kap123 with histone H3 1-28
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2015-11-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | P 1 |
Unit cell lengths | 79.098, 88.114, 102.276 |
Unit cell angles | 79.59, 80.77, 70.74 |
Refinement procedure
Resolution | 44.614 - 2.700 |
R-factor | 0.1863 |
Rwork | 0.185 |
R-free | 0.22970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vch |
RMSD bond length | 0.008 |
RMSD bond angle | 1.016 |
Data scaling software | HKL-2000 (HKL2000_v714-Linux) |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.043 | 0.587 |
Rpim | 0.042 | 0.425 |
Number of reflections | 69157 | 3411 |
<I/σ(I)> | 18.8 | 1.56 |
Completeness [%] | 99.0 | 98.3 |
Redundancy | 4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296.15 | 0.1M sodium cacodylate (pH 6.5), 0.2M sodium acetate, 30% PEG 4000 and 5% Jeffamin M-600 |