5VBB
Human RNA Pseudouridylate Synthase Domain Containing 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-18 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97914 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 55.200, 92.440, 88.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.520 - 1.940 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | DENZO |
Data scaling software | HKL-3000 |
Phasing software | BALBES |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.940 | 5.260 | 1.940 |
Rmerge | 0.083 | 0.056 | 0.809 |
Rmeas | 0.087 | 0.059 | 0.858 |
Rpim | 0.027 | 0.019 | 0.277 |
Total number of observations | 166822 | ||
Number of reflections | 17148 | ||
<I/σ(I)> | 5.1 | ||
Completeness [%] | 99.4 | 98.9 | 96.7 |
Redundancy | 9.7 | 9.3 | 8.3 |
CC(1/2) | 0.999 | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 21% PEG 3350, 0.2 M LiSO4, 0.1 M BisTris pH6.5 |