5VAF
Crystal structure of accessory secretion protein 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2013-06-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | P 1 |
Unit cell lengths | 41.502, 99.897, 179.069 |
Unit cell angles | 100.66, 90.07, 95.78 |
Refinement procedure
Resolution | 97.654 - 2.771 |
R-factor | 0.212 |
Rwork | 0.208 |
R-free | 0.28160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vae |
RMSD bond length | 0.009 |
RMSD bond angle | 1.069 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 97.654 |
High resolution limit [Å] | 2.770 |
Rmerge | 0.166 |
Number of reflections | 66446 |
<I/σ(I)> | 13.63 |
Completeness [%] | 92.7 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 100 mM Bis-Tris Propane pH 9.2 28% PEG6000 |