5VAE
Crystal structure of accessory secretion protein 1 and 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2013-07-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 152.597, 257.294, 217.724 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.900 - 3.106 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.24830 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.270 |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.900 | 3.217 |
High resolution limit [Å] | 3.106 | 3.106 |
Rmerge | 0.145 | 1.654 |
Rpim | 0.427 | |
Number of reflections | 1164890 | |
<I/σ(I)> | 25.19 | 2.35 |
Completeness [%] | 99.5 | 95.98 |
Redundancy | 15.1 | 15.6 |
CC(1/2) | 0.999 | 0.784 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 220 mM Na2HPO4; 20% (w/v) PEG3350 |