5V6K

Crystal Structure of the Second beta-Prism Domain of RbmC from V. cholerae Bound to N-acetylglucosaminyl-beta-1,2-mannose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-01-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	31.940, 38.770, 51.890
Unit cell angles	90.00, 105.26, 90.00

Refinement procedure

Resolution	30.652 - 1.800
R-factor	0.1667
Rwork	0.166
R-free	0.18930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xez
RMSD bond length	0.003
RMSD bond angle	0.586
Data reduction software	MOSFLM (7.2.1)
Data scaling software	Aimless (0.5.31)
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.770	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.077	0.477
Rmeas	0.089	0.608
Rpim	0.044	0.370
Total number of observations	41463	805
Number of reflections	10585	321
<I/σ(I)>	11.4	1.8
Completeness [%]	91.9	47.2
Redundancy	3.9	2.5
CC(1/2)	0.996	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	0.1 M sodium acetate, 2 M sodium sulfate, 1 mM N-acetylglucosaminyl-beta-1,2-mannose