5V1N

DNA polymerase beta substrate complex with 8-oxoG:A at the primer terminus and incoming dCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-18
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	50.546, 79.225, 55.536
Unit cell angles	90.00, 107.32, 90.00

Refinement procedure

Resolution	39.612 - 2.005
R-factor	0.1847
Rwork	0.181
R-free	0.23660
RMSD bond length	0.008
RMSD bond angle	1.009
Data scaling software	HKL-3000
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.005
Number of reflections	51707
<I/σ(I)>	13.9
Completeness [%]	99.4
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	50 mM imidazole, 350 mM sodium acetate, 17% PEG3350