5V1J

DNA polymerase beta open product complex with 8-oxoG:C and inserted dCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-17
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	55.273, 79.795, 55.887
Unit cell angles	90.00, 110.29, 90.00

Refinement procedure

Resolution	31.761 - 2.616
R-factor	0.2242
Rwork	0.217
R-free	0.28520
RMSD bond length	0.009
RMSD bond angle	1.134
Data scaling software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.616
Number of reflections	25305
<I/σ(I)>	14.3
Completeness [%]	99.6
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	50 mM imidazole, 350 mM sodium acetate, 17% PEG3350