5V1F

DNA polymerase beta substrate complex with 8-oxoG at the primer terminus and incoming dCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-31
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	50.218, 79.016, 55.626
Unit cell angles	90.00, 107.01, 90.00

Refinement procedure

Resolution	25.405 - 2.181
R-factor	0.2329
Rwork	0.226
R-free	0.29650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fms
RMSD bond length	0.009
RMSD bond angle	1.062
Data scaling software	HKL-3000
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.181
Number of reflections	36996
<I/σ(I)>	16.5
Completeness [%]	99.4
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	50 mM imidazole, 350 mM sodium chloride, 17% PEG3350