5UZV
Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' recessed-end DNA (rI)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.488, 72.488, 182.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.200 - 2.450 |
R-factor | 0.2166 |
Rwork | 0.213 |
R-free | 0.25390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qea |
RMSD bond length | 0.002 |
RMSD bond angle | 0.482 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1839) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.059 | 0.656 |
Rmeas | 0.064 | 0.716 |
Number of reflections | 18545 | 907 |
<I/σ(I)> | 32.69 | 2.36 |
Completeness [%] | 98.9 | 100 |
Redundancy | 6.3 | 6.4 |
CC(1/2) | 0.830 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000 |