5UMN

Crystal structure of C05 VPGSGW mutant bound to H3 influenza hemagglutinin, HA1 subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 43
Unit cell lengths	88.344, 88.344, 255.038
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	88.340 - 1.970
R-factor	0.18254
Rwork	0.181
R-free	0.20830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 4FP8
RMSD bond length	0.013
RMSD bond angle	1.599
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.400	2.040
High resolution limit [Å]	1.970	1.970
Rpim	0.066	0.353
Number of reflections	136341	13627
<I/σ(I)>	15.4	2
Completeness [%]	99.5	99.5
Redundancy	6.3	6.2
CC(1/2)		0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M sodium citrate pH 5.5 and 9% PEG 8000