5ULM
Structure of the ASK1 central regulatory region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.234, 57.117, 103.575 |
Unit cell angles | 90.00, 104.87, 90.00 |
Refinement procedure
Resolution | 47.100 - 2.100 |
R-factor | 0.23499 |
Rwork | 0.233 |
R-free | 0.26716 |
Structure solution method | SIRAS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.435 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.100 |
High resolution limit [Å] | 2.100 |
Number of reflections | 49197 |
<I/σ(I)> | 8.3 |
Completeness [%] | 99.4 |
Redundancy | 5 |
CC(1/2) | 0.994 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 290 | 0.1 M Sodium Fluoride, 10 % PEG 3350, |