5UL9

Structure and function of the divalent anion/Na+ symporter from Vibrio cholerae and a humanized variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-11
Detector	MAR CCD 130 mm
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	106.125, 102.112, 167.991
Unit cell angles	90.00, 99.52, 90.00

Refinement procedure

Resolution	15.000 - 2.780
R-factor	0.24264
Rwork	0.242
R-free	0.26070
RMSD bond length	0.006
RMSD bond angle	1.051
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.780
Number of reflections	82226
<I/σ(I)>	20
Completeness [%]	93.0
Redundancy	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	PEG, Na+, etc.