5UL7
Structure and function of the divalent anion/Na+ symporter from Vibrio cholerae and a humanized variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-11 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.694, 101.918, 167.755 |
Unit cell angles | 90.00, 98.97, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.26085 |
Rwork | 0.260 |
R-free | 0.27016 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.042 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 82858 |
<I/σ(I)> | 21 |
Completeness [%] | 99.9 |
Redundancy | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | PEG, Na+, etc. |