5UDH
HHARI/ARIH1-UBCH7~Ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-02 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97920 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 184.571, 76.788, 147.724 |
Unit cell angles | 90.00, 107.33, 90.00 |
Refinement procedure
Resolution | 141.010 - 3.240 |
R-factor | 0.22493 |
Rwork | 0.222 |
R-free | 0.27906 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.382 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CRANK |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 141.010 | 3.450 |
High resolution limit [Å] | 3.240 | 3.240 |
Rmerge | 0.093 | 0.883 |
Number of reflections | 31387 | |
<I/σ(I)> | 13.1 | 1.9 |
Completeness [%] | 98.9 | 96 |
Redundancy | 5.8 | 5.9 |
CC(1/2) | 1.000 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 1 ul protein mixture with 10-12 mg/ml protein in 25 mM Tris pH 7.0, 150 mM NaCl, 1 mM DTT and 1 ul well solution with 7-10% PEG 5000 MME, 0.1 M HP pH 7.0 and 5% Tascimate pH 7.0. Over 1 ml reservoir. |