5UCZ
Class II fructose-1,6-bisphosphate aldolase E149A variant of Helicobacter pylori with DHAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-17 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.474, 86.133, 91.196 |
| Unit cell angles | 90.00, 100.28, 90.00 |
Refinement procedure
| Resolution | 29.911 - 1.780 |
| R-factor | 0.1864 |
| Rwork | 0.184 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c4u |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.618 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2481)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.911 |
| High resolution limit [Å] | 1.780 |
| Rmerge | 0.085 |
| Number of reflections | 54638 |
| <I/σ(I)> | 10.86 |
| Completeness [%] | 87.3 |
| Redundancy | 3.3 |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | PEG 8000, PEG 1000, Calcium acetate, Tris-Acetic acid buffer |
