5UBX
Crystal structure of a mutant mIgG2b Fc heterodimer in complex with Protein A peptide analog Z34C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 101.490, 101.490, 135.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.139 - 2.700 |
R-factor | 0.2118 |
Rwork | 0.209 |
R-free | 0.25740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rgs |
RMSD bond length | 0.005 |
RMSD bond angle | 0.858 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (dev_1428) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.053 | 0.802 |
Number of reflections | 19956 | |
<I/σ(I)> | 21.85 | 2.48 |
Completeness [%] | 99.7 | 99 |
Redundancy | 6.33 | 6.37 |
CC(1/2) | 0.999 | 0.813 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 17% PEG 3350, 0.2 M LiCl, 0.1 M Tris, pH 8.0 |