5U6Z
Crystal Structure of Xenopus laevis Apex2 C-terminal Znf-GRF Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-28 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.28257 |
Spacegroup name | I 4 |
Unit cell lengths | 90.332, 90.332, 23.105 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.166 - 2.600 |
R-factor | 0.2 |
Rwork | 0.195 |
R-free | 0.24460 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.460 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
Rmerge | 0.074 | 0.058 | 0.314 |
Number of reflections | 5570 | ||
<I/σ(I)> | 8 | ||
Completeness [%] | 97.9 | 99.3 | 84.4 |
Redundancy | 3.7 | 3.8 | 3.2 |
CC(1/2) | 0.994 | 0.945 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.8 M (NH4)2SO4, 0.1 M Bicine pH 9.0 |