5U4M

RTA-V1C7-G29R-no_salt

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	64.912, 64.912, 215.419
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.836 - 2.500
R-factor	0.2046
Rwork	0.202
R-free	0.24650
RMSD bond length	0.003
RMSD bond angle	0.753
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.080	0.035	0.739
Rmeas	0.084
Rpim	0.026
Total number of observations	195749
Number of reflections	19235
<I/σ(I)>	8.6
Completeness [%]	99.9	99.3	99.7
Redundancy	10.2	9.3	8.1
CC(1/2)		0.999	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	295	8% PEG 4000 and 100 mM sodium acetate pH 4.6