5TYY
DNA Polymerase Mu Product Complex, Mn2+ (60 min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-17 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.985, 68.670, 110.181 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.082 - 1.931 |
R-factor | 0.1874 |
Rwork | 0.186 |
R-free | 0.21790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.958 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.700 |
High resolution limit [Å] | 1.670 | 4.530 | 1.670 |
Rmerge | 0.079 | 0.062 | 0.904 |
Rmeas | 0.087 | ||
Rpim | 0.034 | ||
Total number of observations | 344374 | ||
Number of reflections | 53715 | ||
<I/σ(I)> | 10.6 | ||
Completeness [%] | 99.8 | 98.9 | 99.1 |
Redundancy | 6.4 | 6.2 | 5.6 |
CC(1/2) | 0.994 | 0.739 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG4000 |