5TYD
DNA Polymerase Mu Reactant Complex, 10 mM Mg2+ (45 min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-23 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.891, 68.590, 110.476 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.941 - 1.899 |
R-factor | 0.173 |
Rwork | 0.171 |
R-free | 0.21160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.898 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.899 | 5.160 | 1.900 |
Rmerge | 0.070 | 0.022 | 0.963 |
Number of reflections | 36696 | ||
<I/σ(I)> | 7.4 | ||
Completeness [%] | 100.0 | 99.7 | 100 |
Redundancy | 7.4 | 6.8 | 7.4 |
CC(1/2) | 0.999 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG4000 |