5TY4
MicroED structure of a complex between monomeric TGF-b and its receptor, TbRII, at 2.9 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ELECTRON MICROSCOPE |
Source details | OTHER |
Temperature [K] | 293 |
Detector technology | CMOS |
Collection date | 2016-05-04 |
Detector | TVIPS TEMCAM-F416 |
Wavelength(s) | 0.0250793397 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.530, 71.330, 79.508 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.640 - 2.900 |
R-factor | 0.2948 |
Rwork | 0.292 |
R-free | 0.32800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ktz |
RMSD bond length | 0.012 |
RMSD bond angle | 1.573 |
Data reduction software | MOSFLM (7.1.0) |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX (1.10) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.640 | 3.070 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.293 | 2.024 |
Number of reflections | 3884 | |
<I/σ(I)> | 3.3 | 0.8 |
Completeness [%] | 71.9 | 71.3 |
Redundancy | 3.8 | 3.9 |
CC(1/2) | 0.951 | 0.255 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.5 ul 20 mg/mL protein + 0.25 ul mother liquor + 0.2 ul seed stock in 100 mM HEPES/NaOH pH 7.5, 45% MPD |