5TTR
LEU 55 PRO TRANSTHYRETIN CRYSTAL STRUCTURE
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1995-05 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 149.990, 78.740, 98.950 |
Unit cell angles | 90.00, 100.50, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.700 |
R-factor | 0.199 * |
Rwork | 0.199 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tta |
RMSD bond length | 0.019 |
RMSD bond angle | 0.030 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.055 | 0.353 |
Number of reflections | 31394 | |
<I/σ(I)> | 14.8 | 2.4 |
Completeness [%] | 98.8 | 98.1 |
Redundancy | 3.4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | drop contained 50:50 mixture of protein and reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 45 (%sat) | |
3 | 1 | reservoir | PEG400 | 7 (%) | |
4 | 1 | reservoir | HEPES | 100 (mM) |