5TKF
Neurospora crassa polysaccharide monooxygenase 2 high mannosylation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 43.300, 67.040, 84.150 |
Unit cell angles | 97.57, 97.61, 97.43 |
Refinement procedure
Resolution | 40.308 - 2.100 |
R-factor | 0.1435 |
Rwork | 0.142 |
R-free | 0.17730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eir |
RMSD bond length | 0.003 |
RMSD bond angle | 0.703 |
Data reduction software | MOSFLM (7.2.1) |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX (1.10.1-2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.970 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.088 | 0.303 |
Rmeas | 0.124 | |
Rpim | 0.088 | |
Total number of observations | 102821 | |
Number of reflections | 52135 | |
<I/σ(I)> | 8.8 | |
Completeness [%] | 97.1 | 96.1 |
Redundancy | 2 | 2 |
CC(1/2) | 0.957 | 0.726 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.2 | 295 | PEG 3350, HEPES |