5TDJ

RNA decamer duplex with four 2'-5'-linkages

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	27.538, 94.455, 21.283
Unit cell angles	90.00, 111.19, 90.00

Refinement procedure

Resolution	19.900 - 1.500
R-factor	0.15385
Rwork	0.152
R-free	0.17033
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ms9
RMSD bond length	0.010
RMSD bond angle	1.962
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.064	0.554
Number of reflections	7870
<I/σ(I)>	18.3	1.8
Completeness [%]	96.7	88.7
Redundancy	3.9	3
CC(1/2)		0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	10% MPD (2-methyl-2,4-pentanediol), 40 mM sodium cacodylate pH 7.0, 12 mM spermine tetrahydrochloride, 40mM LiCl, 20 mM MgCl2 and 80 mM SrCl2