5T6J

Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.999965
Spacegroup name	I 41 2 2
Unit cell lengths	85.889, 85.889, 95.003
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.944 - 1.752
R-factor	0.225
Rwork	0.224
R-free	0.24180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ftx
RMSD bond length	0.006
RMSD bond angle	0.793
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.944
High resolution limit [Å]	1.750
Number of reflections	18161
<I/σ(I)>	25.3
Completeness [%]	100.0
Redundancy	17.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES, pH 7.0-7.5, 1.0 M potassium sodium tartrate, 0.2 M lithium sulfate