5T2B
mPI3Kd IN COMPLEX WITH 5e
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.972 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.793, 64.498, 115.976 |
Unit cell angles | 90.00, 103.36, 90.00 |
Refinement procedure
Resolution | 49.930 - 2.300 |
R-factor | 0.224 |
Rwork | 0.222 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.050 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.6) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.370 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.062 | 0.630 |
Number of reflections | 45119 | |
<I/σ(I)> | 11.8 | 1.9 |
Completeness [%] | 97.9 | 96 |
Redundancy | 3.1 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50% ethylene glycol/PEG 8000, 0.1 M carboxylic acids mix, 0.1 M Buffer system 2 pH 7.5 |