5T0Q

Crystal structure of the Myc3 N-terminal domain [44-242] in complex with JAZ10 Jas domain [166-192] from arabidopsis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.078
Spacegroup name	P 32 2 1
Unit cell lengths	86.098, 86.098, 57.871
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.182 - 2.150
R-factor	0.1975
Rwork	0.195
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rqw
RMSD bond length	0.008
RMSD bond angle	1.067
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.220
High resolution limit [Å]	2.150	2.150
Rmerge	0.102	1.000
Number of reflections	13793
<I/σ(I)>	16.3	2.8
Completeness [%]	100.0	100
Redundancy	11.6	11.8
CC(1/2)	0.996	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	20% w/v polyethylene glycol 3,350, 0.2 M magnesium formate dihydrate, pH 7.0.