5SZU
Novel Structural Insights into GDP-Mediated Regulation of Acyl-CoA Thioesterases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9762 |
Spacegroup name | P 21 3 |
Unit cell lengths | 153.198, 153.198, 153.198 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.445 - 2.800 |
R-factor | 0.1723 |
Rwork | 0.170 |
R-free | 0.21380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ien |
RMSD bond length | 0.017 |
RMSD bond angle | 1.567 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.450 | 2.890 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.123 | 0.574 |
Number of reflections | 28972 | |
<I/σ(I)> | 6.45 | 1.96 |
Completeness [%] | 97.4 | 99.66 |
Redundancy | 3.5 | 1.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 100 mM Tris pH 8.5 and 2 M ammonium phosphate |